Sunday, October 24, 2010

Macromolecules

All liveing matter are composed of the following four types of macromolecules:
1)DNA
2)Carbohydrates
3)Lipids
4)Proteins

DNA (Deoxyribonucleic Acids)

-Main purpos: stores genentic material for inheritance, replication, protein synthesisand reproduction.
 -It is a long complex chain, polymer,  made up of a nitrogenous base five-carbon sugar and a phosphate group.
- five nitrogenous bases: adnine, guanine, thymine, uracil, and cytosine (A,T,C,G)
-contains phosphodiester bonds, hydrogen bonds, and glycosyl bonds.
-functional groups are: carboxyl group and hydroxyl group


Carbohydrates

-most common organic materials on Earth.
-formula: (CH2O)
-made up of sugars and their polymers. (OH) group on carbon.
-essential for energy storage and support and structure. Also an energy source for plants and animals.
1) Monosacchride:  (5-6 Carbons)
         -simpleast carbohydrates
         -has linear struture, but through condnsation the chain froms cyclic structures and produce water.
         -Aldose:  has aldehyde funtional group.
                        C=O on the end of the polymer.
         - Ketose:  has keytone functional group.
                        R-C=O on second or the middle of the polymer.
         - glcose and galactose -> form hexagonl rings due to its aldehyde structres)
         -fructose -> forms a pentagonal ring because of its keytone structure.
2) Disacchrides:  
         -two sugars   
         -two types of monosaccharides are covalently linked.
         - maltose (glucose + glucose)
         -sucrose (glucose + frotose)
         -lactose (glactose + glucose)
3) Polysacchride: 
         -forms when thousands of monosaccharide subunites are linked together.        
         -Amylose: (starch) (1-4) linkage
         -Amylopectine:  both (1-4) and (1-6) linkages
         -Glycogen (1-6) linkage
4) Oliaosacchride:  
         -Lectins and selectins



Lipids

-Fats (tryglyceride), Steroids and Phospholipids
-fats: made up of glycerol molecule
-saturated: no double covalent bonds b/w the carbon of the fatty acides. Also has linear structure. (e.g.butter)
-Unsacturated: has ouble bonds with kink. (e.g. margarine)
-phospholipids: bonded to two fatty acids and a phosphate group. The phosphate goup is polar whereas the strands are hydrophobic.
-steriods: eg. cholesterol
-Lipids: good source of energy,and membrane structure, hormones, vitamins.



Proteins

-Numerous different functions and shapes.
1) Primary: the sequence of amino acides linked together by amio group and carboxyl group.
                -peptide linkage.
2)Secondary: Hydrogen bond.
               -bends into alpha helix (coil) or beta sheet (plaitedsheet)
3)Teritary: Combine both coil and sheets. (interactions b/w amino acid side chains) 
                -both ionic and covalent bonds. (Vander wall)
4)Quaternary: more than one teritary linkages come together.
               - combination of more than one proteins.



          

DNA REPLICATION




DNA REPLICATION SUMMARY

-To summarize, DNA replication is a semiconservative process, which means there is one parent strand and one daughter strand in the replicated DNA.
-Each parent strand is a template for ordering nucleotides to make a new complimentary strand.
-There are many sites of replication on a strand of DNA called "replication bubbles", with replication forks on each ends.
-The strands in the double helix are antiparallel, so one strand runs in 5' -> 3' direction, while the other runs in 3'->5' direction  (A new DNA strand can only elongate in the 5'->3' direction)

Leading Strand: (continous replication)
 -form a continuous complimentary strand
 -5' -> 3' using DNA polymerase III.
 -into the fork

Lagging strand:
-From 5' ->3', it forms pieces from Okazaki fragments.
-strand are copied away from the fork.
-First RNA primase lace down the RNA primer, the DNA polymerase III (a.k.a. the little brother) lace down new DNA. This process repeats again and again continously. Then DNA Plymerase I (the bigger brother) replaces RNA primer with DNA. Fnally DNA ligase comes and link OKAZAKI fragmets.
 
 
Definition of key ENZYMES:
 
DNA helicase unwinds the double helix.
DNA gyrase (bacterial enzyme) relieves the tension (produced from unwinding of DNA).
Single-stranded binding proteins (SSBs) keeps separated strands of DNA apart.
Primase (RNA polymerase) makes primer, which signals Polymerase III to make complementary strand.
DNA Polymerase III then grabs nucleotides to make complementary strands of DNA.
DNA Polymerase I then replaces the RNA primer with DNA.
DNA ligase join all the gaps that are present on the daughter strands.



 DNA REPLICATION VIDEO WEBSITES:

1) http://www.youtube.com/watch?v=4jtmOZaIvS0&feature=related2) http://www.youtube.com/watch?v=teV62zrm2P0&feature=related